anti glut1 primary antibody  (Proteintech)

Journal: Poultry Science

Article Title: Chromium picolinate alleviates heat stress-induced breast muscle glucose and lipid metabolism disorders in broiler chickens

doi: 10.1016/j.psj.2025.105704

Figure Lengend Snippet: Effects of chromium picolinate on breast muscle glycolipid metabolism genes expression of broilers under heat stress. (A) Western blot analysis. (B) GLUT1. (C) PI3K. (D) GS. (E) PPARα. (F) CPT-1. (G) LPL. Abbreviation: GLUT1: glucose transporter 1, PI3K: phosphatidylinositol 3 kinase, GS: glycogen synthase, PPARα: peroxisome proliferators-activated receptors α, CPT-1; carnitine palmitoyl transterase-1, LPL: lipoprotein lipase. In the same rank, a, b, c values with different small letter superscripts mean significant difference ( P < 0.05).

Article Snippet: Primary antibodies against GLUT1 (1:1000, AWA61570 , Abiowell), PI3K (1:1000, AWA63394 , Abiowell), GS (1:1000, AWA43660 , Abiowell), CPT1 (1:1000, AWA63836 , Abiowell), LPL (1:1000, AWA61104 , Abiowell), PPARα (1:500, BS-23398, BIOSS) and β-actin (1:2000, AWA80002 ; Abiowell) were diluted in antibody diluent (AWB0200c; Abiowell) and incubated with the membrane overnight at 4°C.

Techniques: Expressing, Western Blot

Journal: The World Allergy Organization Journal

Article Title: Increased glucose transporter 1 contributes to epithelial barrier dysfunction in allergic rhinitis ☆

doi: 10.1016/j.waojou.2025.101158

Figure Lengend Snippet: GLUT1 is elevated in the nasal mucosa and positively correlated with disease severity in AR patients. (A–B) Immunofluorescence staining for GLUT1 (n = 12); (C–D) WB showing GLUT1 protein expression (n = 12); (E) RT-qPCR for GLUT1 (n = 30); (F–G) The correlations between GLUT1 mRNA, VAS and TNSS in AR patients (n = 30). AR, allergic rhinitis; HCs, healthy control; GLUT1, glucose transporter 1; WB, western blotting; RT-qPCR, quantitative reverse transcription polymerase chain reaction; VAS, visual analog scale; TNSS, total nasal symptom score. ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.

Article Snippet: Slides were then incubated overnight at 4 °C with an anti-GLUT1 primary antibody (1:200, Proteintech, Wuhan, China).

Techniques: Immunofluorescence, Staining, Expressing, Quantitative RT-PCR, Control, Western Blot, Reverse Transcription, Polymerase Chain Reaction

Journal: The World Allergy Organization Journal

Article Title: Increased glucose transporter 1 contributes to epithelial barrier dysfunction in allergic rhinitis ☆

doi: 10.1016/j.waojou.2025.101158

Figure Lengend Snippet: Increased GLUT1 is associated with epithelial tight junction marker expression in AR patients. (A–B) Tissue multiplex immunofluorescence for GLUT1, ZO-1, and occluding; (C–D) RT-qPCR for ZO-1 and occluding between the 2 groups (n = 30); (E–F) The correlations between GLUT1 mRNA, ZO-1 and occluding mRNA in AR patients (n = 30). AR, allergic rhinitis; HCs, healthy control; GLUT1, glucose transporter 1; RT-qPCR, quantitative reverse transcription polymerase chain reaction. ∗∗P < 0.01, ∗∗∗P < 0.001.

Article Snippet: Slides were then incubated overnight at 4 °C with an anti-GLUT1 primary antibody (1:200, Proteintech, Wuhan, China).

Techniques: Marker, Expressing, Multiplex Assay, Immunofluorescence, Quantitative RT-PCR, Control, Reverse Transcription, Polymerase Chain Reaction

Journal: The World Allergy Organization Journal

Article Title: Increased glucose transporter 1 contributes to epithelial barrier dysfunction in allergic rhinitis ☆

doi: 10.1016/j.waojou.2025.101158

Figure Lengend Snippet: HDM stimulation promotes GLUT1 expression and inhibits tight junction marker expression in nasal epithelial cells. (A–B) Cell immunofluorescence staining for GLUT1, ZO-1 and occluding in nasal epithelial cells treated with different concentrations of HDM (n = 6); (C–D) WB showing GLUT1, ZO-1 and occluding protein expression in nasal epithelial cells treated with different concentrations of HDM (n = 6). HDM, house dust mite; GLUT1, glucose transporter 1; WB, western blotting. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.

Article Snippet: Slides were then incubated overnight at 4 °C with an anti-GLUT1 primary antibody (1:200, Proteintech, Wuhan, China).

Techniques: Expressing, Marker, Immunofluorescence, Staining, Western Blot

Journal: The World Allergy Organization Journal

Article Title: Increased glucose transporter 1 contributes to epithelial barrier dysfunction in allergic rhinitis ☆

doi: 10.1016/j.waojou.2025.101158

Figure Lengend Snippet: Inhibiting GLUT1 alleviates the suppression of tight junction marker expression in nasal epithelial cells mediated by HDM. (A–B) Cell immunofluorescence staining for GLUT1, ZO-1 and occluding in nasal epithelial cells treated with PBS, HDM and HDM + BAY876 (n = 6); WB showing GLUT1, ZO-1 and occluding protein expression in nasal epithelial cells among the 3 groups (n = 6). HDM, house dust mite; GLUT1, glucose transporter 1; WB, western blotting. ∗P < 0.05, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.

Article Snippet: Slides were then incubated overnight at 4 °C with an anti-GLUT1 primary antibody (1:200, Proteintech, Wuhan, China).

Techniques: Marker, Expressing, Immunofluorescence, Staining, Western Blot

Journal: The World Allergy Organization Journal

Article Title: Increased glucose transporter 1 contributes to epithelial barrier dysfunction in allergic rhinitis ☆

doi: 10.1016/j.waojou.2025.101158

Figure Lengend Snippet: GLUT1 inhibitor alleviates allergen-mediated nasal mucosal inflammation and barrier function damage in murine AR model. (A) Representative HE and immunofluorescence images of nasal mucosal in PBS, AR and AR + BAY876 groups (n = 5); (B) Relative fluorescence intensity of GLUT1, ZO-1 and occluding among the 3 groups (n = 5); (C) Relative fluorescence intensity of GLUT1, ZO-1 and occluding among the 3 groups (n = 5); (D) IL-4, IL-13, IL-17A and IFN-γ concentrations in nasal lavage fluid (n = 5). AR, allergic rhinitis; GLUT1, glucose transporter 1; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

Article Snippet: Slides were then incubated overnight at 4 °C with an anti-GLUT1 primary antibody (1:200, Proteintech, Wuhan, China).

Techniques: Immunofluorescence, Fluorescence

Journal: Frontiers in Physiology

Article Title: Cerebral microvascular density, blood-brain barrier permeability, and support for neuroinflammation indicate early aging in a Marfan syndrome mouse model

doi: 10.3389/fphys.2024.1457034

Figure Lengend Snippet: Representation of neuropathology evaluation. Brains were collected, post-fixed in 4% paraformaldehyde, blocked, sectioned, and stained by Neuroscience Associates (Knoxville, TN). Neuropathology was evaluated by investigating Glut1 staining to evaluate microvascular density, Immunoglobulin G (IgG) staining to assess blood-brain barrier permeability, and iba-1 staining to investigate neuroinflammation in three regions of interest within the hippocampus (dentate gyrus, cornu ammonis 1, and cornu ammonis 3). Created with BioRender.com .

Article Snippet: Glut1, a blood-brain barrier marker, was detected using a rabbit anti-Glut1 primary antibody (Millipore, Catalog# 07-1401; dilution 1:100,000) and a biotinylated goat anti-rabbit IgG secondary antibody (Vector, Catalog# BA-1000; dilution 1:1,000), then visualized using NiDAB as the chromogen.

Techniques: Staining, Permeability

Journal: Frontiers in Physiology

Article Title: Cerebral microvascular density, blood-brain barrier permeability, and support for neuroinflammation indicate early aging in a Marfan syndrome mouse model

doi: 10.3389/fphys.2024.1457034

Figure Lengend Snippet: Glut1 staining in the Dentate Gyrus (DG) is decreased as a function of genotype and age, indicative of decreased microvascular density. (A) Representative images of Glut1 staining in the DG of the hippocampus in 6M-CTRL, 6M-MFS, and 12M-CTRL respectively. Examples of positive signals are represented by white arrows. (B) Glut1 staining is decreased in 6M-MFS DG compared to 6M- CTRL, indicative of decreased microvascular density. 6M-MFS compared to 12M-CTRL mice demonstrate no significant differences in Glut1 staining. Male mice are represented with blue data points and females are represented with red data points.

Article Snippet: Glut1, a blood-brain barrier marker, was detected using a rabbit anti-Glut1 primary antibody (Millipore, Catalog# 07-1401; dilution 1:100,000) and a biotinylated goat anti-rabbit IgG secondary antibody (Vector, Catalog# BA-1000; dilution 1:1,000), then visualized using NiDAB as the chromogen.

Techniques: Staining

Journal: Frontiers in Physiology

Article Title: Cerebral microvascular density, blood-brain barrier permeability, and support for neuroinflammation indicate early aging in a Marfan syndrome mouse model

doi: 10.3389/fphys.2024.1457034

Figure Lengend Snippet: Glut1 staining in the cornu ammonis 1 (CA1) is decreased as a function of genotype and age, indicative of decreased microvascular density. (A) Representative images of Glut1 staining in the CA1 of the hippocampus in 6M-CTRL, 6M-MFS, and 12M-CTRL respectively. Examples of positive signals are represented by white arrows. (B) Glut1 staining is decreased in 6M-MFS CA1 compared to 6M-CTRL. 12M-CTRL CA1 demonstrate decreased Glut1 staining compared to 6M-CTRL, with no differences compared to 6M-MFS mice. Male mice are represented with blue data points and females are represented with red data points.

Article Snippet: Glut1, a blood-brain barrier marker, was detected using a rabbit anti-Glut1 primary antibody (Millipore, Catalog# 07-1401; dilution 1:100,000) and a biotinylated goat anti-rabbit IgG secondary antibody (Vector, Catalog# BA-1000; dilution 1:1,000), then visualized using NiDAB as the chromogen.

Techniques: Staining

Journal: Frontiers in Physiology

Article Title: Cerebral microvascular density, blood-brain barrier permeability, and support for neuroinflammation indicate early aging in a Marfan syndrome mouse model

doi: 10.3389/fphys.2024.1457034

Figure Lengend Snippet: Glut1 staining in the cornu ammonis 3 (CA3) is decreased as a function of genotype and age, indicative of decreased microvascular density. (A) Representative images of Glut1 staining in the CA3 of the hippocampus in 6M-CTRL, 6M-MFS, and 12M-CTRL respectively. Examples of positive signals are represented by white arrows. (B) Glut1 staining is decreased in 6M-MFS CA3 compared to 6M-CTRL. 12M-CTRL CA3 demonstrate decreased Glut1 staining compared to 6M-CTRL, with no differences compared to 6M-MFS mice. Male mice are represented with blue data points and females are represented with red data points.

Article Snippet: Glut1, a blood-brain barrier marker, was detected using a rabbit anti-Glut1 primary antibody (Millipore, Catalog# 07-1401; dilution 1:100,000) and a biotinylated goat anti-rabbit IgG secondary antibody (Vector, Catalog# BA-1000; dilution 1:1,000), then visualized using NiDAB as the chromogen.

Techniques: Staining

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Hypoxia induces a glycolytic complex in intestinal epithelial cells independent of HIF-1-driven glycolytic gene expression.

doi: 10.1073/pnas.2208117120

Figure Lengend Snippet: Fig. 2. Hypoxia increases interactions between proteins involved in glucose metabolism. (A–C) Volcano plots representing fold enrichment (LFQ intensity) of proteins identified in PFKP IP following exposure of cells to 1% O2 for 8 h (A), 24 h (B), or 48 h (C) relative to normoxic PFKP IP. Proteins involved in glucose metabolism indicated in green. (D) Representative immunoblots reflecting HK2 and PFKP expression following co-IP of GLUT1 from whole cell lysates of Caco-2 cells exposed to 21%/1% O2 for 8 to 48 h (Input = 5% total IP).

Article Snippet: Primary antibodies against GLUT1 (CST #12939, 1:1,000 or Invitrogen MA5- 11315, 1:500), HK2 (Invitrogen MA5- 15679, 1:1,000), PFKP (CST #8164, 1:1,000) HIF- 1α (CST #36169, 1:500 or BD Biosciences #610958, 1:500), Nrf2 (CST #12721, 1:1,000), G6PD (Proteintech 25413- 1- AP, 1: 1,000), MTHFD2 (CST #98116, 1:1,000), β- actin (Sigma A5441, 1:5,000) were used alongside speciesspecific HRP- conjugated secondary antibodies [CST #7074 (Anti- Rabbit IgG), #7076 (Anti- Mouse IgG)].

Techniques: Western Blot, Expressing, Co-Immunoprecipitation Assay

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Hypoxia induces a glycolytic complex in intestinal epithelial cells independent of HIF-1-driven glycolytic gene expression.

doi: 10.1073/pnas.2208117120

Figure Lengend Snippet: Fig. 6. Hypoxia increases interactions between cytosolic HIF-1α and proteins involved in glucose metabolism. (A–C) Volcano plots representing fold enrichment (LFQ intensity) of proteins identified in cytosolic HIF-1α IP following exposure of cells to 1% O2 for 8 h (A), 24 h (B), or 48 h (C) relative to normoxic HIF-1α IP (21% O2). Blue, Proteins understood to interact with HIF-1α. Purple, Proteins involved in glucose metabolism (D and E) Representative immunoblots reflecting GLUT1, PFKP, and HIF-1α expression following co-IP of HIF-1α (D) or GLUT1 (E) from cytosolic hypoxic/normoxic wild-type Caco-2 cell fractions or (F) cytosolic HIF-1A NT Caco-2 cell fractions. (G) Cytosolic inputs for (F). Inputs = 10% IP for (D–G).

Article Snippet: Primary antibodies against GLUT1 (CST #12939, 1:1,000 or Invitrogen MA5- 11315, 1:500), HK2 (Invitrogen MA5- 15679, 1:1,000), PFKP (CST #8164, 1:1,000) HIF- 1α (CST #36169, 1:500 or BD Biosciences #610958, 1:500), Nrf2 (CST #12721, 1:1,000), G6PD (Proteintech 25413- 1- AP, 1: 1,000), MTHFD2 (CST #98116, 1:1,000), β- actin (Sigma A5441, 1:5,000) were used alongside speciesspecific HRP- conjugated secondary antibodies [CST #7074 (Anti- Rabbit IgG), #7076 (Anti- Mouse IgG)].

Techniques: Western Blot, Expressing, Co-Immunoprecipitation Assay